《植物生理学报》 2011, 47(6): 570-574

用伊文思蓝染色法检测植物整体叶片的细胞活性

刘楠, 林植芳*

中国科学院华南植物园, 中国科学院退化生态系统植被恢复与管理重点实验室, 广州510650

通信作者：林植芳；Tel: 020-37252995

摘　要：

本文利用伊文思蓝(evans blue)作为细胞活性染料, 检测了胁迫处理后3种草本和2种灌木植物叶片的细胞活性。结果 显示, 不同类型的胁迫(温度、PEG、Pb、Cd、NaCl和NaHSO3)均造成了叶片细胞一定程度的伤害。随着各种胁迫的加剧, 叶片染色面积和染色后提取液吸光度都呈现梯度增加的趋势。研究分别利用染色面积和其提取液吸光度表达叶片细胞的活性, 提出了一种更安全、更准确的植物整体叶片细胞活性检测方法。

关键词：伊文思蓝; 拟南芥; 细胞活性; 整体叶片检测

收稿：2011-03-16   修定：2011-04-20

资助：教育部留学回国人员科研启动基金、广东省科技计划项 目(2010B060200039)和广州市科技亚运专项行动计划项 目(2010u1-E00601)。

Use of Evans Blue for Testing Cell Viability of Intact Leaves of Plant

Liu Nan, LiN Zhi-Fang*

Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China

Corresponding author: LiN Zhi-Fang; Tel: 020-37252995

Abstract:

The current study tested stress induced cell viability of intact leaves of three grass species and two shrub species by evans blue method. The results showed that different types of stresses (temperature, PEG, Pb, Cd, NaCl and NaHSO₃) caused leaf cell damages to different degrees. Along with the exaggeration of different stress treatments, the dyed area in leaves and the extract solution absorbance from dyed leaves were increased in gradient. This study compared two different methods in calculating leaf cell viability by leaf dyed area and extract solution absorbance from dyed leaves, respectively. it also proposed a safer and more accurate method for testing cell viability of intact leaf of plant.

Key words: evans blue; Arabidopsis thaliana; cell viability; intact leaf test